Plasma Membrane–Associated ROP10 Small GTPase Is a Specific Negative Regulator of Abscisic Acid Responses in Arabidopsis
Copyright policy )Plasma Membrane–Associated ROP10 Small GTPase Is a Specific Negative Regulator of Abscisic Acid Responses in Arabidopsis
Abscisic acid (ABA) is an important plant hormone that modulates seed germination and plant growth and stress responses, but its signaling remains poorly understood. We investigated the role of ROP10, a member of the Arabidopsis Rop subfamily of Rho GTPases, in ABA signaling. A null rop10 mutant exhibits enhanced responses to ABA in seed germination, root elongation, and stomatal closure assays and in the induction of expression of the transcription factor MYB2, but it shows wild-type levels of ABA and normal responses to other hormones. Consistently, transgenic expression of a constitutively active form of ROP10 reduces ABA inhibition of seed germination, whereas dominant-negative mutants of ROP10 enhance ABA response and partially suppress abi2. Furthermore, ABA specifically downregulates ROP10 transcription in root tips. ROP10 is localized to the plasma membrane (PM), and PM localization is crucial for its function. These results suggest that ROP10 is a PM-localized signaling molecule that is involved specifically in the negative regulation of ABA signaling.
- University of California System United States
- Indiana University – Purdue University Indianapolis United States
- Purdue University West Lafayette United States
- University of California, San Diego United States
- University of California, San Diego United States
rho GTP-Binding Proteins, Arabidopsis Proteins, Arabidopsis, Membrane Proteins, Germination, Plants, Genetically Modified, Plant Roots, Gene Expression Regulation, Enzymologic, GTP Phosphohydrolases, Plant Growth Regulators, GTP-Binding Proteins, Gene Expression Regulation, Plant, Mutation, Seeds, Phytochrome, Abscisic Acid, Signal Transduction
rho GTP-Binding Proteins, Arabidopsis Proteins, Arabidopsis, Membrane Proteins, Germination, Plants, Genetically Modified, Plant Roots, Gene Expression Regulation, Enzymologic, GTP Phosphohydrolases, Plant Growth Regulators, GTP-Binding Proteins, Gene Expression Regulation, Plant, Mutation, Seeds, Phytochrome, Abscisic Acid, Signal Transduction
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).135 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!