GATC-specific restriction and modification systems in treponemes
Copyright policy )GATC-specific restriction and modification systems in treponemes
To investigate the presence of GATC-specific modification and restriction activities in rumen isolates of Treponema sp.The presence of N6-methyladenine within GATC (Dam) sequences was analysed using isoschizomeric restriction endonucleases having different sensitivities to the methylation of the target sequence. A fast screening method was used for testing of site-specific endonuclease activities directly in crude cell extracts. Three out of six rumen isolates of Treponema sp. showed restriction activities. Restriction endonucleases were further purified by Heparin-Sepharose chromatography. Using PCR and specific primers, no sequence homologous to the T. pallidum dam gene was found.Three rumen treponemal strains were documented to possess MboI isoschizomeric restriction-modification systems.This is the first report on restriction activity in rumen treponemes.
- Polish Academy of Learning Poland
- Kielanowski Institute of Animal Physiology and Nutrition Poland
- Slovak Academy of Sciences Slovakia
- Institute of Chemistry Slovakia
- Institute of Animal Physiology Slovakia
Site-Specific DNA-Methyltransferase (Adenine-Specific), Rumen, Base Sequence, Adenine, Sepharose, Polymerase Chain Reaction, Chromatography, Affinity, Substrate Specificity, Animals, DNA Restriction-Modification Enzymes, Treponema, Deoxyribonucleases, Type II Site-Specific
Site-Specific DNA-Methyltransferase (Adenine-Specific), Rumen, Base Sequence, Adenine, Sepharose, Polymerase Chain Reaction, Chromatography, Affinity, Substrate Specificity, Animals, DNA Restriction-Modification Enzymes, Treponema, Deoxyribonucleases, Type II Site-Specific
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