Increased GABAB Receptor Subtype Expression in the Nucleus of the Solitary Tract of the Spontaneously Hypertensive Rat
Copyright policy )Increased GABAB Receptor Subtype Expression in the Nucleus of the Solitary Tract of the Spontaneously Hypertensive Rat
Expression of GABA(B) receptor messenger RNA (mRNA) in the central nervous system was compared between the spontaneously hypertensive (SHR) and normotensive Wistar Kyoto (WKY) rat. Polymerase chain reaction (PCR) revealed all the isoforms except B1e in cortex, hypothalamus, and medulla oblongata. In the nucleus of the solitary tract (NTS) and ventrolateral medulla (VLM), the B1a-c and 1 g isoforms were present as well as B2. Real-time PCR detected significantly higher levels of B1a (p < 0.01) and B2 (p < 0.05) mRNA in the NTS of SHR compared to WKY. A significant increase in B1a expression (p < 0.05) was detected in VLM. Immunolabeling suggested presynaptic and postsynaptic expression of B1a, B1b, and B2 subtypes throughout the NTS, with significant differences in distribution patterns and labeling between subtypes and between SHR and WKY. These findings suggest that GABA(B) receptors expressed by neurones in NTS may be involved in cardiovascular regulation and that changes in GABA(B) mRNA expression levels may contribute to the hypertensive state in SHR.
- University of Leeds United Kingdom
Male, Neurons, Medulla Oblongata, Reverse Transcriptase Polymerase Chain Reaction, Body Weight, Hypothalamus, Gene Expression, Blood Pressure, Immunohistochemistry, Rats, Alternative Splicing, Protein Subunits, Receptors, GABA-B, Antibody Specificity, Rats, Inbred SHR, Hypertension, Animals, RNA, Messenger, Rats, Wistar, Microscopy, Immunoelectron
Male, Neurons, Medulla Oblongata, Reverse Transcriptase Polymerase Chain Reaction, Body Weight, Hypothalamus, Gene Expression, Blood Pressure, Immunohistochemistry, Rats, Alternative Splicing, Protein Subunits, Receptors, GABA-B, Antibody Specificity, Rats, Inbred SHR, Hypertension, Animals, RNA, Messenger, Rats, Wistar, Microscopy, Immunoelectron
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).15 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!