To evaluate the effect on intraocular pressure (IOP) of increased corneal thickness after Descemet's stripping automated endothelial keratoplasty (DSAEK) and of non-Descemet's stripping automated endothelial keratoplasty (nDSAEK) as measured by four different techniques.Twenty-four eyes (22 patients; mean age, 74.0 years) with successful DSAEK (11 eyes) or nDSAEK (13 eyes) treatment at least 3 months prior to testing were enrolled. IOP was measured with Goldmann applanation tonometry (GAT), dynamic contour tonometry (DCT), pneumatonometry, and Tono-Pen XL (Tonopen). Central corneal thickness (CCT) was measured by ultrasonic pachymetry. These data were used for statistical analysis.Mean IOP measured by GAT, DCT, pneumatonometry, and Tonopen was 14.4, 13.9, 11.2, and 13.2 mmHg, respectively, in the DSAEK group; and 15.0, 14.4, 12.5, and 14.4 mmHg, respectively, in the nDSAEK group. Correlations between IOP and CCT were not statistically significant in either group. Pressure measured by pneumatonometry was significantly and consistently lower than that obtained by the other three methods.For both DSAEK and nDSAEK, IOP readings by the four tonometers seem to be unrelated to artificially thickened corneas.