Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
Copyright policy )Systematic Genetic Analysis with Ordered Arrays of Yeast Deletion Mutants
In Saccharomyces cerevisiae , more than 80% of the ∼6200 predicted genes are nonessential, implying that the genome is buffered from the phenotypic consequences of genetic perturbation. To evaluate function, we developed a method for systematic construction of double mutants, termed synthetic genetic array (SGA) analysis, in which a query mutation is crossed to an array of ∼4700 deletion mutants. Inviable double-mutant meiotic progeny identify functional relationships between genes. SGA analysis of genes with roles in cytoskeletal organization ( BNI1 , ARP2 , ARC40 , BIM1 ), DNA synthesis and repair ( SGS1 , RAD27 ), or uncharacterized functions ( BBC1 , NBP2 ) generated a network of 291 interactions among 204 genes. Systematic application of this approach should produce a global map of gene function.
- Mount Sinai Hospital United States
- McGill University Canada
- Queen's University Canada
- University of Toronto Canada
- Lunenfeld-Tanenbaum Research Institute Canada
Genetic Markers, Endodeoxyribonucleases, Genes, Essential, DNA Repair, Flap Endonucleases, Genes, Fungal, DNA Helicases, Cell Polarity, Computational Biology, Cell Cycle Proteins, Fungal Proteins, Cytoskeletal Proteins, Genetic Techniques, Databases, Genetic, Genome, Fungal, Carrier Proteins, DNA, Fungal, Crosses, Genetic, Cytoskeleton, Gene Deletion
Genetic Markers, Endodeoxyribonucleases, Genes, Essential, DNA Repair, Flap Endonucleases, Genes, Fungal, DNA Helicases, Cell Polarity, Computational Biology, Cell Cycle Proteins, Fungal Proteins, Cytoskeletal Proteins, Genetic Techniques, Databases, Genetic, Genome, Fungal, Carrier Proteins, DNA, Fungal, Crosses, Genetic, Cytoskeleton, Gene Deletion
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