cDNA Cloning, Genomic Organization, and Chromosomal Localization of the Mouse LIM Motif-Containing Kinase Gene, Limk2
Copyright policy )cDNA Cloning, Genomic Organization, and Chromosomal Localization of the Mouse LIM Motif-Containing Kinase Gene, Limk2
LIM-kinases, including LIMK1 and LIMK2, are unique LIM-family proteins with two tandem LIM motifs at the N-terminal and a serine/threonine kinase domain at the C-terminal. In this study, we cloned two types of mouse Limk2 cDNA; one is an intact form (Limk2a) and the other has only one complete LIM domain (Limk2b). Northern blot analysis showed Limk2a mRNA was ubiquitously present in various adult tissues, while Limbk2b was predominantly expressed in brain. We also identified genomic organization of the Limk2 gene; it is similar to that of the related gene Limk1. The transcription unit contains 16 exons plus two alternative exons, thus, variation in the initiator exon usage gives rise to alternative transcripts of Limk2. Fluorescent in situ hybridization analysis showed the Limk2 gene were mapped to mouse chromosome 1D. These findings provide some important clues to the in vivo functions of LIM kinases.
- Hokkaido Bunkyo University Japan
- Hokkaido University Japan
- Osaka University Japan
Genomic Library, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Chromosome Mapping, Lim Kinases, Zinc Fingers, Exons, Protein Serine-Threonine Kinases, Introns, DNA-Binding Proteins, Mice, Animals, Amino Acid Sequence, Cloning, Molecular, In Situ Hybridization, Fluorescence
Genomic Library, DNA, Complementary, Base Sequence, Sequence Homology, Amino Acid, Molecular Sequence Data, Chromosome Mapping, Lim Kinases, Zinc Fingers, Exons, Protein Serine-Threonine Kinases, Introns, DNA-Binding Proteins, Mice, Animals, Amino Acid Sequence, Cloning, Molecular, In Situ Hybridization, Fluorescence
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).25 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Average
Citations provided by BIP!Popularity provided by BIP!