Callus has been derived from Carnation leaves, on (MS) medium, Containing Various Concentrations of plant growth regulators, 0.1 mg/L BA and 0.5 mg/L 2,4-D. Established callus showed a good ability fordifferentiation and shoot formation on MS medium supplemented with 3.0 mg/L BA and 0.1 mg/L IAA at a rate of 100%. Different rooting concentrations were used with IBA in MS medium, 100% rooting wasobtained when IBA used at 1.0 mg/L. Phenols were separated from crude alcoholic extractions from leaves of seed plants, callus of leaves and leaves derived from regeneration plants. Phenols were identified by using ferric chloride reagent. Treatment of both Fusarium oxysporum and Fusarium Culum with these extractions was successful in retarding the growth of both fungi, by means of dry weight. The best crudealcoholic extract in retarding the growth of both fungi was found to be the callus of leaves extracts which caused 67.7% and 62.1 retardation in growth of both fungi Fusarium oxysporum and Fusarium culum respectively.