cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse: comparison with the acatalasemic mutant
Copyright policy )cDNA cloning and expression of mutant catalase from the hypocatalasemic mouse: comparison with the acatalasemic mutant
Mutant catalase cDNAs from the hypocatalasemic and acatalasemic mice were cloned and expressed in bacteria. A novel missense mutation, Asp (AAT) to Ser (AGT), was identified at amino acid position 439 of the hypocatalasemic catalase. Analysis of recombinant catalase mutants revealed that the mutation is responsible for the reduced activity of hypocatalasemic catalase and the unstable tetrameric structure of acatalasemic catalase was also suggested.
- Okayama University Japan
Male, DNA, Complementary, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Catalase, Gene Expression Regulation, Enzymologic, Recombinant Proteins, Acatalasia, Mice, Liver, Mutation, Escherichia coli, Animals, RNA, Cloning, Molecular
Male, DNA, Complementary, Reverse Transcriptase Polymerase Chain Reaction, Molecular Sequence Data, Catalase, Gene Expression Regulation, Enzymologic, Recombinant Proteins, Acatalasia, Mice, Liver, Mutation, Escherichia coli, Animals, RNA, Cloning, Molecular
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