Next-generation sequencing to generate interactome datasets
Copyright policy )Next-generation sequencing to generate interactome datasets
Next-generation sequencing has not been applied to protein-protein interactome network mapping so far because the association between the members of each interacting pair would not be maintained in en masse sequencing. We describe a massively parallel interactome-mapping pipeline, Stitch-seq, that combines PCR stitching with next-generation sequencing and used it to generate a new human interactome dataset. Stitch-seq is applicable to various interaction assays and should help expand interactome network mapping.
- Dana-Farber Cancer Institute United States
- Harvard University United States
- Harvard Medical School United States
- Cornell University United States
- Center for Systems Biology United States
Open Reading Frames, Two-Hybrid System Techniques, Protein Interaction Mapping, Humans, Sequence Analysis, DNA, Databases, Protein, Polymerase Chain Reaction
Open Reading Frames, Two-Hybrid System Techniques, Protein Interaction Mapping, Humans, Sequence Analysis, DNA, Databases, Protein, Polymerase Chain Reaction
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