A novel intermembrane space–targeting signal docks cysteines onto Mia40 during mitochondrial oxidative folding
Copyright policy )A novel intermembrane space–targeting signal docks cysteines onto Mia40 during mitochondrial oxidative folding
Mia40 imports Cys-containing proteins into the mitochondrial intermembrane space (IMS) by ensuring their Cys-dependent oxidative folding. In this study, we show that the specific Cys of the substrate involved in docking with Mia40 is substrate dependent, the process being guided by an IMS-targeting signal (ITS) present in Mia40 substrates. The ITS is a 9-aa internal peptide that (a) is upstream or downstream of the docking Cys, (b) is sufficient for crossing the outer membrane and for targeting nonmitochondrial proteins, (c) forms an amphipathic helix with crucial hydrophobic residues on the side of the docking Cys and dispensable charged residues on the other side, and (d) fits complementary to the substrate cleft of Mia40 via hydrophobic interactions of micromolar affinity. We rationalize the dual function of Mia40 as a receptor and an oxidase in a two step–specific mechanism: an ITS-guided sliding step orients the substrate noncovalently, followed by docking of the substrate Cys now juxtaposed to pair with the Mia40 active Cys.
Protein Folding, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Calorimetry, Protein Sorting Signals, Mitochondrial Membrane Transport Proteins, Mitochondrial Proteins, Copper Transport Proteins, Consensus Sequence, Mitochondrial Precursor Protein Import Complex Proteins, Humans, Cysteine, Cation Transport Proteins, Research Articles, Binding Sites, BLUE NATIVE ELECTROPHORESIS; MEMBRANE-PROTEIN COMPLEXES; DISULFIDE RELAY SYSTEM; YEAST MITOCHONDRIA; SORTING SIGNAL; IN-VIVO; IMPORT; CYTOCHROME-B2;, Mitochondria, Protein Structure, Tertiary, BLUE NATIVE ELECTROPHORESIS; MEMBRANE-PROTEIN COMPLEXES; DISULFIDE RELAY SYSTEM; YEAST MITOCHONDRIA; SORTING SIGNAL; IN-VIVO; IMPORT; CYTOCHROME-B2, Protein Transport, Carrier Proteins, Oxidation-Reduction, Molecular Chaperones
Protein Folding, Saccharomyces cerevisiae Proteins, Saccharomyces cerevisiae, Calorimetry, Protein Sorting Signals, Mitochondrial Membrane Transport Proteins, Mitochondrial Proteins, Copper Transport Proteins, Consensus Sequence, Mitochondrial Precursor Protein Import Complex Proteins, Humans, Cysteine, Cation Transport Proteins, Research Articles, Binding Sites, BLUE NATIVE ELECTROPHORESIS; MEMBRANE-PROTEIN COMPLEXES; DISULFIDE RELAY SYSTEM; YEAST MITOCHONDRIA; SORTING SIGNAL; IN-VIVO; IMPORT; CYTOCHROME-B2;, Mitochondria, Protein Structure, Tertiary, BLUE NATIVE ELECTROPHORESIS; MEMBRANE-PROTEIN COMPLEXES; DISULFIDE RELAY SYSTEM; YEAST MITOCHONDRIA; SORTING SIGNAL; IN-VIVO; IMPORT; CYTOCHROME-B2, Protein Transport, Carrier Proteins, Oxidation-Reduction, Molecular Chaperones
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