The taxonomic status of ‘Actinoplanes liguriae’ A/6353 and ‘Actinoplanes teichomyceticus’ AB8327 was established by using a polyphasic approach. Strains A/6353 and AB8327 form distinct phylogenetic lineages in the 16S rRNA gene tree of members of the genus Actinoplanes and are related moderately and closely to Actinoplanes rectilineatus and Actinoplanes cyaneus, respectively. Morphological, cultural and physiological properties indicated that strains A/6353 and AB8327 represent separate, novel species of the genus Actinoplanes, Actinoplanes liguriensis sp. nov. (type strain A/6353T=FH 2244T=DSM 43865T=ATCC 31048T=BCRC 12121T=CBS 355.75T=IMSNU 22127T=JCM 3250T=KCTC 9536T=KCC A-0250T=NBRC 13997T=NCIMB 12636T=NRRL B-16723T=SANK 62178T) and Actinoplanes teichomyceticus sp. nov. (type strain AB8327T=FH 2149T=DSM 43866T=ATCC 31121T=BCRC 12106T=FERM P-3462T=IMSNU 20043T=IMET 9254T=JCM 3252T=KCC A-0252T=KCTC 9543T=NBRC 13999T=NCIMB 12640T=NRRL B-16726T=SANK 60479T).