Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
Copyright policy )Rho-GTPase–dependent filamentous actin dynamics coordinate vesicle targeting and exocytosis during tip growth
The dynamic activity of tip-localized filamentous actin (F-actin) in pollen tubes is controlled by counteracting RIC4 and RIC3 pathways downstream of the ROP1 guanosine triphosphatase promoting actin assembly and disassembly, respectively. We show here that ROP1 activation is required for both the polar accumulation and the exocytosis of vesicles at the plasma membrane apex. The apical accumulation of exocytic vesicles oscillated in phase with, but slightly behind, apical actin assembly and was enhanced by overexpression of RIC4. However, RIC4 overexpression inhibited exocytosis, and this inhibition could be suppressed by latrunculin B treatment or RIC3 overexpression. We conclude that RIC4-dependent actin assembly is required for polar vesicle accumulation, whereas RIC3-mediated actin disassembly is required for exocytosis. Thus ROP1-dependent F-actin dynamics control tip growth through spatiotemporal coordination of vesicle targeting and exocytosis.
- University of Michigan–Flint United States
- University of California System United States
- University of California, Riverside United States
- University of California, San Francisco United States
- University of Michigan United States
Nicotiana, rho GTP-Binding Proteins, 570, Recombinant Fusion Proteins, Pollen Tube, Medical and Health Sciences, Models, Biological, Exocytosis, Models, GTP-Binding Proteins, 616, Tobacco, Transport Vesicles, Research Articles, Arabidopsis Proteins, Biological Sciences, Biological, Actins, Enzyme Activation, Actin Cytoskeleton, Protein Transport, Carrier Proteins, Developmental Biology, Fluorescence Recovery After Photobleaching, Subcellular Fractions
Nicotiana, rho GTP-Binding Proteins, 570, Recombinant Fusion Proteins, Pollen Tube, Medical and Health Sciences, Models, Biological, Exocytosis, Models, GTP-Binding Proteins, 616, Tobacco, Transport Vesicles, Research Articles, Arabidopsis Proteins, Biological Sciences, Biological, Actins, Enzyme Activation, Actin Cytoskeleton, Protein Transport, Carrier Proteins, Developmental Biology, Fluorescence Recovery After Photobleaching, Subcellular Fractions
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