The Fetal Liver Counterpart of Adult Common Lymphoid Progenitors Gives Rise to All Lymphoid Lineages, CD45+CD4+CD3− Cells, As Well As Macrophages
Copyright policy )The Fetal Liver Counterpart of Adult Common Lymphoid Progenitors Gives Rise to All Lymphoid Lineages, CD45+CD4+CD3− Cells, As Well As Macrophages
Abstract We identified an IL-7Rα+Sca-1lowc-Kitlow population in E14 fetal liver, which is the phenotypical analog of common lymphoid progenitors (CLP) in adult bone marrow. After transfer into newborn mice, the IL-7Rα+Sca-1lowc-Kitlow population rapidly differentiated into CD45+CD4+CD3− cells, which are candidate cells for initiating lymph node and Peyer’s patch formation. In addition, this population also gave rise to B, T, NK, and CD8α+ and CD8α− dendritic cells. The fetal liver precursors expressed a significantly lower level of the myeloid-suppressing transcription factor Pax-5, than adult CLP, and retained differentiation activity for macrophages in vitro. We propose that the transition from fetal liver IL-7Rα+Sca-1lowc-Kitlow cells to adult CLP involves a regulated restriction of their developmental potential, controlled, at least in part, by Pax-5 expression.
- Vrije Universiteit Amsterdam Netherlands
- Stanford University United States
- Amsterdam UMC, location VUmc Netherlands
- Amsterdam UMC Netherlands
- Erasmus University Rotterdam Netherlands
CD3 Complex, B-Lymphocytes/cytology, Fetus/cytology, Membrane Proteins/biosynthesis, Inbred C57BL, Cell Differentiation/immunology, Mice, Receptors, Stem Cells/cytology, Antigens, Ly, CD4 Antigens/biosynthesis, Myeloid Cells/cytology, Cells, Cultured, Erythroid Precursor Cells, B-Lymphocytes, Cultured, Newborn/immunology, Gene Expression Regulation, Developmental, Cell Differentiation, Liver, Leukocyte Common Antigens/biosynthesis, CD4 Antigens, Cells, Liver/cytology, Lymphocyte Subsets/cytology, Immunophenotyping, Fetus, Ly/biosynthesis, Animals, Cell Lineage, Antigens, CD3 Complex/biosynthesis, Developmental/immunology, Macrophages, Macrophages/cytology, Dendritic Cells, Interleukin-7/biosynthesis, Lymphocyte Subsets, Liver Transplantation, Lymph Nodes/cytology, Cell Lineage/immunology, Erythroid Precursor Cells/cytology, Proto-Oncogene Proteins c-kit/biosynthesis, Gene Expression Regulation, Animals, Newborn, Liver Transplantation/immunology, Leukocyte Common Antigens, Lymph Nodes, Dendritic Cells/transplantation, Stem Cell Transplantation
CD3 Complex, B-Lymphocytes/cytology, Fetus/cytology, Membrane Proteins/biosynthesis, Inbred C57BL, Cell Differentiation/immunology, Mice, Receptors, Stem Cells/cytology, Antigens, Ly, CD4 Antigens/biosynthesis, Myeloid Cells/cytology, Cells, Cultured, Erythroid Precursor Cells, B-Lymphocytes, Cultured, Newborn/immunology, Gene Expression Regulation, Developmental, Cell Differentiation, Liver, Leukocyte Common Antigens/biosynthesis, CD4 Antigens, Cells, Liver/cytology, Lymphocyte Subsets/cytology, Immunophenotyping, Fetus, Ly/biosynthesis, Animals, Cell Lineage, Antigens, CD3 Complex/biosynthesis, Developmental/immunology, Macrophages, Macrophages/cytology, Dendritic Cells, Interleukin-7/biosynthesis, Lymphocyte Subsets, Liver Transplantation, Lymph Nodes/cytology, Cell Lineage/immunology, Erythroid Precursor Cells/cytology, Proto-Oncogene Proteins c-kit/biosynthesis, Gene Expression Regulation, Animals, Newborn, Liver Transplantation/immunology, Leukocyte Common Antigens, Lymph Nodes, Dendritic Cells/transplantation, Stem Cell Transplantation
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).223 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 1% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 1%
Citations provided by BIP!Popularity provided by BIP!