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Simultaneous Purification of Limonin, Nomilin and Isoobacunoic Acid from Pomelo Fruit (Citrus grandis) Segment Membrane

descriptionPublicationkeyboard_double_arrow_right Article 11 Sep 2014 English Publisher:WileyJournal:Journal of Food Science, volume 79 (issn: 0022-1147, eissn: 1750-3841,

Authors: Yu Xiang; Kunsong Chen; Fenglei Luo; Jinping Cao; Xian Li; Dengliang Wang; Wei Chen; +1 Authors

doi: 10.1111/1750-3841.12581

pmid: 25212475

Simultaneous Purification of Limonin, Nomilin and Isoobacunoic Acid from Pomelo Fruit (Citrus grandis) Segment Membrane

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Abstract

AbstractA method was established for purification of limonin, nomilin, and isoobacunoic acid simultaneously from segment membranes of pomelo (Citrus Grandis). This method includes 3 steps, removing most impurities by macroporous resin HZ‐816, isolating limonin by High Speed Counter Current Chromatography (HSCCC), and isolating nomilin and isoobacunoic acid by semi‐preparative HPLC. Naringin was partially purified as a by‐product of this process using Sephadex LH‐20. All limonoids purified through this method reached 95% purity. The purified limonin, nomilin and isoobacunoic acid were identified according to the retention time of the standard substances using HPLC and characteristic fragment ions of LC‐MS/MS.

Related Organizations

Zhejiang Ocean University
China (People's Republic of)
Crops Research Institute
Ghana

Keywords

Limonins, Citrus, Mass Spectrometry, Tandem Mass Spectrometry, Fruit, Flavanones, Benzoxepins, Chromatography, Liquid

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