AbstractBosniak renal cyst classification has been widely used in determining the complexity of a renal cyst. However, it turns out that about half of patients undergoing surgery for Bosniak category III, take surgical risks that reward them with no clinical benefit at all. This is because their pathological results reveal that the cysts are actually benign not malignant. This problem inspires us to use recently popular deep learning techniques and study alternative analytics methods for precise binary classification (benign or malignant tumor) on Computerized Tomography (CT) images. To achieve our goal, two consecutive steps are required–segmenting kidney organs or lesions from CT images then classifying the segmented kidneys. In this paper, we propose a study of kidney segmentation using 2.5D ResUNet and 2.5D DenseUNet for efficiently extracting intra-slice and inter-slice features. Our models are trained and validated on the public data set from Kidney Tumor Segmentation (KiTS19) challenge in two different training environments. As a result, all experimental models achieve high mean kidney Dice scores of at least 95% on the KiTS19 validation set consisting of 60 patients. Apart from the KiTS19 data set, we also conduct separate experiments on abdomen CT images of four Thai patients. Based on the four Thai patients, our experimental models show a drop in performance, where the best mean kidney Dice score is 87.60%.