Identification of BOIP, a novel cDNA highly expressed during spermatogenesis that encodes a protein interacting with the orange domain of the hairy‐related transcription factor HRT1/Hey1 in Xenopus and mouse
Copyright policy )Identification of BOIP, a novel cDNA highly expressed during spermatogenesis that encodes a protein interacting with the orange domain of the hairy‐related transcription factor HRT1/Hey1 in Xenopus and mouse
AbstractHairy‐related transcription factor (HRT/Hey) genes encode a novel subfamily of basic helix‐loop‐helix (bHLH) transcription factors related to the Drosophila hairy and Enhancer‐of‐split (E(spl)) and the mammalian HES proteins that function as downstream mediators of Notch signaling. Using the yeast two‐hybrid approach, a previously uncharacterized protein was identified in Xenopus that interacts with XHRT1 (originally referred to as bc8), one member of the HRT/Hey subclass. This protein is evolutionarily conserved in chordates. It binds to sequences adjacent to the bHLH domain of XHRT1 known as the Orange domain and has been named bc8 Orange interacting protein (BOIP). BOIP shows a rather uniform subcellular localization and is recruited to the nucleus upon binding to XHRT1. In Xenopus, XBOIP mRNA is detected by RNase protection analysis throughout embryogenesis. In the adult, the strongest expression is detected in testis. In the mouse, high levels of BOIP mRNA are also found in adult testis. No expression is detected in the embryo and in any of the other adult organs tested. In situ hybridization revealed that BOIP transcripts were detected almost exclusively in round spermatids and that this expression overlaps with that of Hey1 (HRT1), which is expressed throughout spermatogenesis. In view of the importance of the Orange domain for HRT/Hey function, the newly identified BOIP proteins may serve as regulators specifically of HRT1/Hey1 activity. Developmental Dynamics 228:716–725, 2003. © 2003 Wiley‐Liss, Inc.
- University Hospital Würzburg Germany
- University of Würzburg Germany
- IBM (Belgium) Belgium
- Université Libre de Bruxelles Belgium
Ribonucleases -- metabolism, Male, Embryo, Nonmammalian, Sequence Homology, Spermatozoa -- metabolism, Cell Cycle Proteins, Mice, Testis -- metabolism, Receptors, Basic Helix-Loop-Helix Transcription Factors, Developmental, Membrane Proteins -- metabolism, Cloning, Molecular, Luciferases, Conserved Sequence, In Situ Hybridization, Nonmammalian, Gene Expression Regulation, Developmental, Mammalian -- metabolism, Immunohistochemistry, Amino Acid, Embryo, Plasmids -- metabolism, Complementary -- metabolism, Protein Binding, Signal Transduction, RNA -- metabolism, Protein Structure, Notch, DNA, Complementary, Carrier Proteins -- biosynthesis, Molecular Sequence Data, Transfection, Cell Cycle Proteins -- metabolism, Nuclear Proteins -- biosynthesis, Two-Hybrid System Techniques, Animals, Humans, Amino Acid Sequence, Spermatogenesis, Gene Library, Biologie moléculaire, Molecular, Membrane Proteins, DNA, Embryo, Mammalian, Precipitin Tests, Cell Cycle Proteins -- chemistry, Messenger -- metabolism, Gene Expression Regulation, Hela Cells, Carrier Proteins -- genetics, RNA, Carrier Proteins, Luciferases -- metabolism, Nuclear Proteins -- genetics, Tertiary, Cloning, HeLa Cells
Ribonucleases -- metabolism, Male, Embryo, Nonmammalian, Sequence Homology, Spermatozoa -- metabolism, Cell Cycle Proteins, Mice, Testis -- metabolism, Receptors, Basic Helix-Loop-Helix Transcription Factors, Developmental, Membrane Proteins -- metabolism, Cloning, Molecular, Luciferases, Conserved Sequence, In Situ Hybridization, Nonmammalian, Gene Expression Regulation, Developmental, Mammalian -- metabolism, Immunohistochemistry, Amino Acid, Embryo, Plasmids -- metabolism, Complementary -- metabolism, Protein Binding, Signal Transduction, RNA -- metabolism, Protein Structure, Notch, DNA, Complementary, Carrier Proteins -- biosynthesis, Molecular Sequence Data, Transfection, Cell Cycle Proteins -- metabolism, Nuclear Proteins -- biosynthesis, Two-Hybrid System Techniques, Animals, Humans, Amino Acid Sequence, Spermatogenesis, Gene Library, Biologie moléculaire, Molecular, Membrane Proteins, DNA, Embryo, Mammalian, Precipitin Tests, Cell Cycle Proteins -- chemistry, Messenger -- metabolism, Gene Expression Regulation, Hela Cells, Carrier Proteins -- genetics, RNA, Carrier Proteins, Luciferases -- metabolism, Nuclear Proteins -- genetics, Tertiary, Cloning, HeLa Cells
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