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License: CC BY
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image/svg+xml art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos Open Access logo, converted into svg, designed by PLoS. This version with transparent background. http://commons.wikimedia.org/wiki/File:Open_Access_logo_PLoS_white.svg art designer at PLoS, modified by Wikipedia users Nina, Beao, JakobVoss, and AnonMoos http://www.plos.org/
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Image . 2011
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(A–D) MDCK/AQP2 cells were transfected with TM5b siRNA (B) or scrambled control siRNA (A), which was indicated by fluorescein-labeled dsRNA (red), and treated without (top) or with (bottom) forskolin

appsOther research productkeyboard_double_arrow_right Image 01 Jan 2011Publisher:figshare
Authors: Noda, Yumi; Horikawa, Saburo; Kanda, Eiichiro; Yamashita, Maho; Meng, Hu; Eto, Kayoko; Li, Yuhua; +6 Authors

doi: 10.6084/m9.figshare.86772.v1 , 10.6084/m9.figshare.86772

(A–D) MDCK/AQP2 cells were transfected with TM5b siRNA (B) or scrambled control siRNA (A), which was indicated by fluorescein-labeled dsRNA (red), and treated without (top) or with (bottom) forskolin

Abstract

Pretreatment with H89 was also performed using the cells transfected with TM5b siRNA (D) or the control siRNA (C). Cells were labeled for AQP2 (green). Bars, 10 μm. (E and F) Apical cell surface biotinylation assay using MDCK/AQP2 cells transfected with TM5b siRNA or control siRNA, corresponding to A–D. (E) Biotinylated proteins were precipitated with streptavidin-agarose beads and immunoblotted for AQP2. (F) The densitometric quantification normalized to control siRNA-transfected cells without forskolin treatment. Data represent the mean and SE from three independent experiments. *, P < 0.05 versus control siRNA-transfected cells without forskolin treatment; **, P < 0.05 versus control siRNA-transfected cells pretreated with H89 without forskolin stimulation; ***, P < 0.05 versus control siRNA-transfected cells pretreated with H89 with forskolin stimulation.Copyright information:Taken from "Reciprocal interaction with G-actin and tropomyosin is essential for aquaporin-2 trafficking"The Journal of Cell Biology 2008;182(3):587-601.Published online 11 Aug 2008PMCID:PMC2500142.

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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Average
Average
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