DNA Sequence Motifs Which Direct Adeno-Associated Virus Site-Specific Integration in a Model System
Copyright policy )DNA Sequence Motifs Which Direct Adeno-Associated Virus Site-Specific Integration in a Model System
ABSTRACT The DNA sequence motifs which direct adeno-associated virus type 2 site-specific integration are being investigated using a shuttle vector, propagated as a stable episome in cultured cell lines, as the target for integration. Previously, we reported that the minimum episomal targeting elements comprise a 16-bp binding motif (Rep binding site [RBS]) for a viral regulatory protein (Rep) separated by a short DNA spacer from a sequence (terminal resolution site [TRS]) that can serve as a substrate for Rep-mediated nicking activity (R. M. Linden, P. Ward, C. Giraud, E. Winocour, and K. I. Berns, Proc. Natl. Acad. Sci. USA 93:11288–11294, 1996; R. M. Linden, E. Winocour, and K. I. Berns, Proc. Natl. Acad. Sci. USA 93:7966–7972, 1996). We now report that episomal integration depends upon both the sequence and the position of the spacer DNA separating the RBS and TRS motifs. The spacer thus constitutes a third element required for site-specific episomal integration.
- Weizmann Institute of Science Israel
- University of Florida United States
- Harvard University United States
Binding Sites, Virus Integration, DNA Helicases, Dependovirus, Models, Biological, DNA-Binding Proteins, Viral Proteins, DNA, Viral, Mutagenesis, Site-Directed, Humans, Cell Line, Transformed
Binding Sites, Virus Integration, DNA Helicases, Dependovirus, Models, Biological, DNA-Binding Proteins, Viral Proteins, DNA, Viral, Mutagenesis, Site-Directed, Humans, Cell Line, Transformed
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