P-glycoprotein (encoded by multidrug resistance genes) is not required for interleukin-2 secretion in mice and humans
Copyright policy )P-glycoprotein (encoded by multidrug resistance genes) is not required for interleukin-2 secretion in mice and humans
P-glycoprotein (encoded by multidrug resistance genes), a member of the ATP-binding cassette transporter protein superfamily, has been shown to play a role in the secretion of cytokines. This conclusion was based upon the inhibition of cytokine secretion by anti-P-gp monoclonal antibodies. In this study, we show that anti-CD3-stimulated lymphocytes from wild-type, mdr1a knock out and mdr1ab double knock out mice produce similar amounts of IL-2, IFN-gamma, IL-4, and IL-10. In addition, Jurkat T cells that lack P-gp and MDR1-transfected Jurkat T cells (JurkatP-gp) as well as purified human peripheral blood CD4+ P-gp+ and CD4+ P-gp- and CD8+ P-gp+ and CD8+ P-gp- T cell subsets produced comparable amounts of IL-2. These data show that P-gp is not required for secretion of IL-2, IFN-gamma, IL-4, and IL-10 secretion in mice and IL-2 secretion in humans.
- University of California, Irvine United States
CD4-Positive T-Lymphocytes, Male, Mice, Knockout, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, DNA, CD8-Positive T-Lymphocytes, In Vitro Techniques, Interleukin-10, Interferon-gamma, Jurkat Cells, Mice, Animals, Humans, Interleukin-2, ATP Binding Cassette Transporter, Subfamily B, Member 1, Interleukin-4, Genes, MDR, Cell Division, DNA Primers
CD4-Positive T-Lymphocytes, Male, Mice, Knockout, Base Sequence, Reverse Transcriptase Polymerase Chain Reaction, DNA, CD8-Positive T-Lymphocytes, In Vitro Techniques, Interleukin-10, Interferon-gamma, Jurkat Cells, Mice, Animals, Humans, Interleukin-2, ATP Binding Cassette Transporter, Subfamily B, Member 1, Interleukin-4, Genes, MDR, Cell Division, DNA Primers
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