Activation of naïve CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling
Copyright policy )Activation of naïve CD4 T cells by anti-CD3 reveals an important role for Fyn in Lck-mediated signaling
Although there was no impairment in IL-2 secretion and proliferation of Fyn-deficient naïve CD4 cells after stimulation with antigen and antigen-presenting cells, stimulation of these cells with anti-CD3 and anti-CD28 revealed profound defects. Crosslinking of purified wild-type naïve CD4 cells with anti-CD3 activated Lck and initiated the signaling cascade downstream of Lck, including phosphorylation of ZAP-70, LAT, and PLC-γ1; calcium flux; and dephosphorylation and nuclear translocation of the nuclear factor of activated T cells (NFAT)p. All of these signaling events were diminished severely in Fyn-deficient naïve cells activated by CD3 crosslinking. Coaggregation of CD3 and CD4 reconstituted this Lck-dependent signaling pathway in Fyn -/- T cells. These results suggest that when signaling of naïve T cells is restricted to the T cell antigen receptor, Fyn plays an essential role by positive regulation of Lck activity.
- La Jolla Institute For Allergy & Immunology United States
CD4-Positive T-Lymphocytes, Mice, Knockout, CD3 Complex, Cell Culture Techniques, Receptors, Antigen, T-Cell, Mice, Transgenic, Lymphocyte Activation, Transcription Factor AP-1, Mice, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Animals, Interleukin-2, Calcium, Lymph Nodes, Phosphorylation, Spleen, Adaptor Proteins, Signal Transducing
CD4-Positive T-Lymphocytes, Mice, Knockout, CD3 Complex, Cell Culture Techniques, Receptors, Antigen, T-Cell, Mice, Transgenic, Lymphocyte Activation, Transcription Factor AP-1, Mice, Lymphocyte Specific Protein Tyrosine Kinase p56(lck), Animals, Interleukin-2, Calcium, Lymph Nodes, Phosphorylation, Spleen, Adaptor Proteins, Signal Transducing
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).53 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!