Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix
Copyright policy )Dynamics of transcription-mediated conversion from euchromatin to facultative heterochromatin at the Xist promoter by Tsix
The fine-scale dynamics from euchromatin (EC) to facultative heterochromatin (fHC) has remained largely unclear. Here, we focus on Xist and its silencing initiator Tsix as a paradigm of transcription-mediated conversion from EC to fHC. In mouse epiblast stem cells, induction of Tsix recapitulates the conversion at the Xist promoter. Investigating the dynamics reveals that the conversion proceeds in a stepwise manner. Initially, a transient opened chromatin structure is observed. In the second step, gene silencing is initiated and dependent on Tsix, which is reversible and accompanied by simultaneous changes in multiple histone modifications. At the last step, maintenance of silencing becomes independent of Tsix and irreversible, which correlates with occupation of the −1 position of the transcription start site by a nucleosome and initiation of DNA methylation introduction. This study highlights the hierarchy of multiple chromatin events upon stepwise gene silencing establishment.
Cell Reports, 34 (13)
ISSN:2666-3864
ISSN:2211-1247
- ETH Zurich Switzerland
- RIKEN Center for Biosystems Dynamics Research Japan
- Hamamatsu University School of Medicine Japan
- École Polytechnique Fédérale de Lausanne EPFL Switzerland
- Institute of Science Tokyo Japan
CCCTC-Binding Factor, Transcription, Genetic, QH301-705.5, local gene silencing, Epigenesis, Genetic, Euchromatin, Histones, Mice, cis-NATs, Heterochromatin, Animals, Gene Silencing, Biology (General), Promoter Regions, Genetic, YY1 Transcription Factor, epigenetics, Stem Cells, dynamics, DNA Methylation, Fibroblasts, Tsix, Nucleosomes, RNA, Long Noncoding, Protein Processing, Post-Translational, Germ Layers
CCCTC-Binding Factor, Transcription, Genetic, QH301-705.5, local gene silencing, Epigenesis, Genetic, Euchromatin, Histones, Mice, cis-NATs, Heterochromatin, Animals, Gene Silencing, Biology (General), Promoter Regions, Genetic, YY1 Transcription Factor, epigenetics, Stem Cells, dynamics, DNA Methylation, Fibroblasts, Tsix, Nucleosomes, RNA, Long Noncoding, Protein Processing, Post-Translational, Germ Layers
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