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Regulation of the yeast HIS7 gene by the global transcription factor Abf1p

descriptionPublicationkeyboard_double_arrow_right Article 01 Sep 1997Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 256, pages 136-146 (issn: 0026-8925, eissn: 1432-1874,

Authors: Markus Künzler; Sven Krappmann; C. Zmasek; Christoph Springer; Gerhard H. Braus;

doi: 10.1007/s004380050555

pmid: 9349705

Regulation of the yeast HIS7 gene by the global transcription factor Abf1p

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Abstract

The HIS7 gene of Saccharomyces cerevisiae encodes a bifunctional glutamine amidotransferase: cyclase that catalyzes the formation of biosynthetic precursors for histidine and adenine. HIS7 is activated by Gcn4p upon amino acid starvation and by the Bas1/2p complex in response to adenine limitation. Mutation analysis of the HIS7 promoter in a deltagcn4 background revealed a polyd(A/T) stretch and a d(CT) repeat as essential elements for Gcn4p-independent basal HIS7 transcription. The protein binding this element was enriched and identified as the multifunctional DNA-binding protein Abf1p. Abf1p binds specifically to the d(CT) repeat sequence, which represents a novel Abf1p-binding motif, and protects 17 nucleotides from digestion by DNase I. In addition, Abf1p binding causes bending of the HIS7 promoter structure.

Related Organizations

University of Göttingen
Germany

Keywords

Saccharomyces cerevisiae Proteins, Poly T, Saccharomyces cerevisiae, Polymerase Chain Reaction, DNA-Binding Proteins, Fungal Proteins, Lac Operon, Aminohydrolases, Multienzyme Complexes, Gene Expression Regulation, Fungal, Protein Biosynthesis, Mutagenesis, Site-Directed, Deoxyribonuclease I, Cloning, Molecular, DNA, Fungal, Dinucleotide Repeats, Poly A, Promoter Regions, Genetic, Protein Kinases, Repetitive Sequences, Nucleic Acid

5 Research products, page 1 of 1

Myb-like DNA-binding protein BAS1
2017IsRelatedTo
ARS-binding factor 1
2017IsRelatedTo
Regulatory protein PHO2
2017IsRelatedTo
General control protein GCN4
2017IsRelatedTo
Imidazole glycerol phosphate synthase hisHF
2017IsRelatedTo

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