p95-APP1 links membrane transport to Rac-mediated reorganization of actin
Copyright policy )p95-APP1 links membrane transport to Rac-mediated reorganization of actin
Motility requires protrusive activity at the cellular edge, where Rho family members regulate actin dynamics. Here we show that p95-APP1 (ArfGAP-putative, Pix-interacting, paxillin-interacting protein 1), a member of the GIT1/PKL family, is part of a complex that interacts with Rac. Wild-type and truncated p95-APP1 induce actin-rich protrusions mediated by Rac and ADP-ribosylation factor 6 (Arf6). Distinct p95-APP1-derived polypeptides have different distributions, indicating that p95-APP1 cycles between the cell surface and endosomes. Our results show that p95-APP1 functionally interacts with Rac and localizes to endosomal compartments, thus identifying p95-APP1 as a molecular link between actin organization, adhesion, and membrane transport during cell motility.
- National Research Council Italy
- University of Southern Denmark Denmark
- Vita-Salute San Raffaele University Italy
- National Academies of Sciences, Engineering, and Medicine United States
- Odense University Denmark
Cells, Recombinant Fusion Proteins, Molecular Sequence Data, Fluorescent Antibody Technique, Cell Cycle Proteins, Chick Embryo, Endosomes, Models, Biological, Chromatography, Affinity, Models, Cell Movement, Cell Adhesion, Animals, Amino Acid Sequence, Cloning, Molecular, Cells, Cultured, Cytoskeleton, Adaptor Proteins, Signal Transducing, Chromatography, Cultured, ADP-Ribosylation Factors, GTPase-Activating Proteins, Cell Membrane, Signal Transducing, Adaptor Proteins, Molecular, Biological Transport, Fibroblasts, Biological, Phosphoproteins, Precipitin Tests, Actins, rac GTP-Binding Proteins, Molecular Weight, Enzyme Activation, Affinity, ADP-Ribosylation Factor 6, Guanosine Triphosphate, Carrier Proteins, Sequence Alignment, Cloning, Protein Binding
Cells, Recombinant Fusion Proteins, Molecular Sequence Data, Fluorescent Antibody Technique, Cell Cycle Proteins, Chick Embryo, Endosomes, Models, Biological, Chromatography, Affinity, Models, Cell Movement, Cell Adhesion, Animals, Amino Acid Sequence, Cloning, Molecular, Cells, Cultured, Cytoskeleton, Adaptor Proteins, Signal Transducing, Chromatography, Cultured, ADP-Ribosylation Factors, GTPase-Activating Proteins, Cell Membrane, Signal Transducing, Adaptor Proteins, Molecular, Biological Transport, Fibroblasts, Biological, Phosphoproteins, Precipitin Tests, Actins, rac GTP-Binding Proteins, Molecular Weight, Enzyme Activation, Affinity, ADP-Ribosylation Factor 6, Guanosine Triphosphate, Carrier Proteins, Sequence Alignment, Cloning, Protein Binding
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