Apurinic endonuclease from Drosophila melanogaster: Reduced enzymatic activity in excision-deficient mutants of the mei-9 and mus(2)201 loci

descriptionPublicationkeyboard_double_arrow_right Article 01 Jul 1982 English Publisher:Springer Science and Business Media LLCJournal:Molecular and General Genetics MGG, volume 186, pages 235-239 (issn: 0026-8925, eissn: 1432-1874,

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Authors: C J, Osgood; J B, Boyd;

doi: 10.1007/bf00331855

pmid: 6287173

Apurinic endonuclease from Drosophila melanogaster: Reduced enzymatic activity in excision-deficient mutants of the mei-9 and mus(2)201 loci

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Abstract

An endonuclease activity, which is specific for partially depurinated PM2 DNA, has been assayed in extracts prepared from cultured cells and larval brain ganglia of Drosophila melanogaster. Activity detected in repair-proficient cells is stimulated by Mg++ and is inhibited by EDTA. Extracts prepared from established cell cultures of the excision-deficient strain mei-9a and from larval brain ganglia derived from y mei-9a, mei-9L1, sn3 mei-9D2 and sn3 mei-9D4 are partially deficient in this activity. At least one allele of a second excision-deficient strain, cn mus (2)201D1, also shows reduced AP-endonuclease activity in ganglia extracts.

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University of California, Davis
United States

Keywords

Apurinic Acid, Deoxyribonucleases, DNA Repair, Endonucleases, Deoxyribonuclease IV (Phage T4-Induced), Drosophila melanogaster, Culture Techniques, Mutation, DNA-(Apurinic or Apyrimidinic Site) Lyase, Animals, Ganglia, Cells, Cultured

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