Sen34p depletion blocks tRNA splicing in vivo and delays rRNA processing
Copyright policy )Sen34p depletion blocks tRNA splicing in vivo and delays rRNA processing
Tif6p (eIF6) is necessary for 60S biogenesis, rRNA maturation and must be released from 60S to permit 80S assembly and translation. We characterized Tif6p interactors. Tif6p is mostly on 66S-60S pre-ribosomes, partly free. Tif6p complex(es) contain nucleo-ribosomal factors and Asc1p. Surprisingly, Tif6p particle contains the low-abundance endonuclease Sen34p. We analyzed Sen34p role on rRNA/tRNA synthesis, in vivo. Sen34p depletion impairs tRNA splicing and causes unexpected 80S accumulation. Accordingly, Sen34p overexpression causes 80S decrease and increased polysomes which suggest increased translational efficiency. With delayed kinetics, Sen34p depletion impairs rRNA processing. We conclude that Sen34p is absolutely required for tRNA splicing and that it is a rate-limiting element for efficient translation. Finally, we confirm that Tif6p accompanies 27S pre-rRNA maturation to 25S rRNA and we suggest that Sen34p endonuclease in Tif6p complex may affect also rRNA maturation.
- University of Milano-Bicocca Italy
- Wellcome Centre for Cell Biology United Kingdom
- University of Edinburgh United Kingdom
- University of Milan Italy
- Carnegie Mellon University United States
Ribosomal Proteins, Saccharomyces cerevisiae Proteins, RNA Splicing, Saccharomyces cerevisiae, Phosphoproteins, Kinetics, Intermediate Filament Proteins, RNA, Transfer, RNA, Ribosomal, Gene Expression Regulation, Fungal, Endoribonucleases, RNA Precursors, 60S; eIF3; eIF6 (alias p27BBP); Pre-rRNA; RACK1; Carrier Proteins; Endoribonucleases; Gene Deletion; Gene Expression Regulation, Fungal; Intermediate Filament Proteins; Kinetics; Phosphoproteins; RNA Precursors; RNA, Ribosomal; RNA, Transfer; Ribosomal Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; RNA Processing, Post-Transcriptional; RNA Splicing; Biochemistry; Biophysics; Molecular Biology, RNA Processing, Post-Transcriptional, Carrier Proteins, eIF6 (alias p27BBP); Pre-rRNA; 60S; eIF3; RACK1, Gene Deletion
Ribosomal Proteins, Saccharomyces cerevisiae Proteins, RNA Splicing, Saccharomyces cerevisiae, Phosphoproteins, Kinetics, Intermediate Filament Proteins, RNA, Transfer, RNA, Ribosomal, Gene Expression Regulation, Fungal, Endoribonucleases, RNA Precursors, 60S; eIF3; eIF6 (alias p27BBP); Pre-rRNA; RACK1; Carrier Proteins; Endoribonucleases; Gene Deletion; Gene Expression Regulation, Fungal; Intermediate Filament Proteins; Kinetics; Phosphoproteins; RNA Precursors; RNA, Ribosomal; RNA, Transfer; Ribosomal Proteins; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins; RNA Processing, Post-Transcriptional; RNA Splicing; Biochemistry; Biophysics; Molecular Biology, RNA Processing, Post-Transcriptional, Carrier Proteins, eIF6 (alias p27BBP); Pre-rRNA; 60S; eIF3; RACK1, Gene Deletion
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