Arbitrary Priming PCR Shows Randomly Distributed Transposon Insertions Among 19 Mutant Clones and Identifies a cylE Insertional Knockout. Geneious alignment image from 19 Sanger sequencing reactions performed from APPCR on individual mutant A2 library clones, showing random distribution along the A909 genome (green bar at bottom) and no siblings with identical insertions (A). Expected orientation of transposon in the cylE insertional knockout, with confirmatory PCR primer locations and agarose gel of confirmatory PCR (B). Pigment phenotype of WT A909 and cylE insertional knockout grown in new Granada media (C). (PDF 1154 kb)