The Lymphotoxin LTα1β2 Controls Postnatal and Adult Spleen Marginal Sinus Vascular Structure and Function
Copyright policy )The Lymphotoxin LTα1β2 Controls Postnatal and Adult Spleen Marginal Sinus Vascular Structure and Function
The lymphotoxin LTalpha(1)beta(2) supports the development and maintenance of several aspects of spleen structure, but its significance for marginal sinus (MS) vascular organization is unclear. We showed here that, in early postnatal lymphotoxin-deficient mice, the developing Flk-1+ white pulp vessels failed to organize or upregulate MAdCAM-1, leading to altered spatial rearrangement of both the white pulp endothelial cells and the smooth muscle actin-expressing cells. In vitro, MAdCAM-1 directed the reorganization of LTbeta receptor+ endothelial cells grown on Matrigel. LTalpha(1)beta(2) also regulated the maintenance of both MAdCAM-1 expression and mature MS structure in adult mice, contributing importantly to normal trafficking of CD11b+ cells in response to bacterial antigens. Together, our studies demonstrate that LTalpha(1)beta(2) and LTbeta receptor signals control proper development and maintenance of the mature MS structure and implicate MAdCAM-1 in the structuring of the MS endothelial cells that is important for the movement of immune cells within the spleen.
- University of Alabama at Birmingham United States
- University of Alabama at Birmingham, Department of Microbiology United States
- University of Mary United States
- Medical College of Wisconsin United States
- Department of Microbiology United States
Mice, Knockout, Antigens, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, Immunology, Lymphotoxin alpha1, beta2 Heterotrimer, Endothelial Cells, Enzyme-Linked Immunosorbent Assay, Cadherins, Cell Line, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Mice, Infectious Diseases, Mucoproteins, CELLIMMUNO, Antigens, CD, Immunology and Allergy, Animals, Cell Adhesion Molecules, Cells, Cultured, Spleen, Signal Transduction
Mice, Knockout, Antigens, Bacterial, Reverse Transcriptase Polymerase Chain Reaction, Immunology, Lymphotoxin alpha1, beta2 Heterotrimer, Endothelial Cells, Enzyme-Linked Immunosorbent Assay, Cadherins, Cell Line, Mice, Inbred C57BL, Platelet Endothelial Cell Adhesion Molecule-1, Mice, Infectious Diseases, Mucoproteins, CELLIMMUNO, Antigens, CD, Immunology and Allergy, Animals, Cell Adhesion Molecules, Cells, Cultured, Spleen, Signal Transduction
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