Integration host factor positively regulates cysJIH transcription
Copyright policy )Integration host factor positively regulates cysJIH transcription
We report the identification of integration host factor (IHF) as an additional element involved in the regulation of the cysJIH promoter of Salmonella typhimurium and Escherichia coli. An IHF-binding site was located in the regulatory region of the cysJIH promoter by using two methods, protection against DNase I digestion and hydroxyl radical cleavage. The positive influence of IHF on in vitro run-off transcription from the cysJIH promoter is shown. The in vivo observations suggest that IHF is necessary for full expression of cysJIH in stationary phase but not during exponential growth.
- Polish Academy of Learning Poland
- Kazan Institute of Biochemistry and Biophysics Russian Federation
Integration Host Factors, Salmonella typhimurium, Binding Sites, Base Sequence, Molecular Sequence Data, Bacterial Proteins, Gene Expression Regulation, Mutation, Escherichia coli, Cysteine, Promoter Regions, Genetic
Integration Host Factors, Salmonella typhimurium, Binding Sites, Base Sequence, Molecular Sequence Data, Bacterial Proteins, Gene Expression Regulation, Mutation, Escherichia coli, Cysteine, Promoter Regions, Genetic
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