N4WBP5, a Potential Target for Ubiquitination by the Nedd4 Family of Proteins, Is a Novel Golgi-associated Protein
Copyright policy )N4WBP5, a Potential Target for Ubiquitination by the Nedd4 Family of Proteins, Is a Novel Golgi-associated Protein
Nedd4 belongs to a family of ubiquitin-protein ligases that is characterized by 2--4 WW domains, a carboxyl-terminal Hect (homologous to E6-AP Carboxyl terminus)domain and in most cases an amino-terminal C2 domain. We had previously identified a series of proteins that associates with the WW domains of Nedd4. In this paper, we demonstrate that one of the Nedd4-binding proteins, N4WBP5, belongs to a small group of evolutionarily conserved proteins with three transmembrane domains. N4WBP5 binds Nedd4 WW domains via the two PPXY motifs present in the amino terminus of the protein. In addition to Nedd4, N4WBP5 can interact with the WW domains of a number of Nedd4 family members and is ubiquitinated. Endogenous N4WBP5 localizes to the Golgi complex. Ectopic expression of the protein disrupts the structure of the Golgi, suggesting that N4WBP5 forms part of a family of integral Golgi membrane proteins. Based on previous observations in yeast, we propose that N4WBP5 may act as an adaptor for Nedd4-like proteins and their putative targets to control ubiquitin-dependent protein sorting and trafficking.
- University of Queensland Australia
- University of Queensland Australia
- University of Adelaide Australia
- University of Queensland Australia
- South Australia Pathology Australia
Biochemistry & Molecular Biology, Identification, 570, Proline, 572, Nedd4 Ubiquitin Protein Ligases, Ubiquitin-Protein Ligases, Messenger, Ligase Nedd4, Molecular Sequence Data, Golgi Apparatus, Expression, 270100 Biochemistry and Cell Biology, Transfection, Ligases, Degradation, Mice, C1, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Ww Domains, Epithelial Na+ Channel, Liddles-syndrome, Endosomal Sorting Complexes Required for Transport, Ubiquitin, Calcium-Binding Proteins, Membrane Proteins, Binding, 730118 Organs, diseases and abnormal conditions not elsewhere classified, RNA, Intercellular Signaling Peptides and Proteins, Carrier Proteins, Transcription
Biochemistry & Molecular Biology, Identification, 570, Proline, 572, Nedd4 Ubiquitin Protein Ligases, Ubiquitin-Protein Ligases, Messenger, Ligase Nedd4, Molecular Sequence Data, Golgi Apparatus, Expression, 270100 Biochemistry and Cell Biology, Transfection, Ligases, Degradation, Mice, C1, Animals, Humans, Amino Acid Sequence, RNA, Messenger, Ww Domains, Epithelial Na+ Channel, Liddles-syndrome, Endosomal Sorting Complexes Required for Transport, Ubiquitin, Calcium-Binding Proteins, Membrane Proteins, Binding, 730118 Organs, diseases and abnormal conditions not elsewhere classified, RNA, Intercellular Signaling Peptides and Proteins, Carrier Proteins, Transcription
selected citations These citations are derived from selected sources.This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).108 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!