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Immunobiology
Article . 2020 . Peer-reviewed
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Immunobiology
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Immunobiology
Article . 2020
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Immunobiology
Article . 2020
License: Elsevier TDM
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Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2)

Authors: Sarkar, Bishajit; Ullah, Md. Asad; Johora, Fatema Tuz; Taniya, Masuma Afrin; Araf, Yusha;

Immunoinformatics-guided designing of epitope-based subunit vaccines against the SARS Coronavirus-2 (SARS-CoV-2)

Abstract

SARS Coronavirus-2 (SARS-CoV-2) pandemic has become a global issue which has raised the concern of scientific community to design and discover a counter-measure against this deadly virus. So far, the pandemic has caused the death of hundreds of thousands of people upon infection and spreading. To date, no effective vaccine is available which can combat the infection caused by this virus. Therefore, this study was conducted to design possible epitope-based subunit vaccines against the SARS-CoV-2 virus using the approaches of reverse vaccinology and immunoinformatics. Upon continual computational experimentation, three possible vaccine constructs were designed and one vaccine construct was selected as the best vaccine based on molecular docking study which is supposed to effectively act against the SARS-CoV-2. Thereafter, the molecular dynamics simulation and in silico codon adaptation experiments were carried out in order to check biological stability and find effective mass production strategy of the selected vaccine. This study should contribute to uphold the present efforts of the researches to secure a definitive preventative measure against this lethal disease.

Keywords

COVID-19 Vaccines, Protein Conformation, Immunology, Pneumonia, Viral, Epitopes, T-Lymphocyte, Article, Betacoronavirus, Epitopes, Immunogenicity, Vaccine, HLA Antigens, Immunology and Allergy, Humans, Amino Acid Sequence, Pandemics, COVID-19, Computational Biology, Hematology, Reverse Genetics, Molecular Docking Simulation, Host-Pathogen Interactions, Disease Progression, Epitopes, B-Lymphocyte, Coronavirus Infections, Plasmids

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    82
    popularity
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    Top 1%
    influence
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    Top 10%
    impulse
    This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
    Top 1%
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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
82
Top 1%
Top 10%
Top 1%
Green
gold