Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors
Copyright policy )Evaluation of mRNA by Q-RTPCR and protein expression by AQUA of the M2 subunit of ribonucleotide reductase (RRM2) in human tumors
The purpose of this study was to evaluate baseline RRM2 protein and gene expression in tumors of patients receiving 3-AP.Tumor blocks from patients enrolled in phase I and II clinical studies using 3-AP, were evaluated for RRM2 gene and protein expression by quantitative real time polymerase chain reaction (Q-RTPCR) and automated quantitative analysis (AQUA).Esophageal and gastric cancers overexpressed RRM2 protein when compared to prostate cancer (Z-score, 0.68 +/- 0.94 SD, vs 0.41 +/- 0.84 SD, respectively; p = 0.04). Esophageal and gastric cancers also overexpressed RRM2 mRNA when compared to prostate cancer (relative gene expression 2.56 +/- 1.49 SD, vs 0.29 +/- 0.20 SD, respectively; p = 0.02). Protein and gene expression were moderately associated (Spearman's rank correlation = 0.30; p = 0.12).RRM2 gene and protein expression varies by tumor type.
- University of Wisconsin System United States
- University of Wisconsin–Oshkosh United States
- National Cancer Institute United States
- National Institute of Health Pakistan
- University of Wisconsin–Madison United States
Male, Thiosemicarbazones, Clinical Trials, Phase I as Topic, Esophageal Neoplasms, Ribonucleoside Diphosphate Reductase, Pyridines, Prostatic Neoplasms, Polymerase Chain Reaction, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Protein Subunits, Clinical Trials, Phase II as Topic, Stomach Neoplasms, Humans, RNA, Messenger
Male, Thiosemicarbazones, Clinical Trials, Phase I as Topic, Esophageal Neoplasms, Ribonucleoside Diphosphate Reductase, Pyridines, Prostatic Neoplasms, Polymerase Chain Reaction, Gene Expression Regulation, Enzymologic, Gene Expression Regulation, Neoplastic, Protein Subunits, Clinical Trials, Phase II as Topic, Stomach Neoplasms, Humans, RNA, Messenger
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