Hepatocyte nuclear factor 4 alpha is related to survival of the condensed mesenchyme in the developing mouse kidney
Copyright policy )Hepatocyte nuclear factor 4 alpha is related to survival of the condensed mesenchyme in the developing mouse kidney
AbstractHepatocyte nuclear factor 4 alpha (Hnf4α) is a transcription factor required for embryogenesis and organogenesis. In the adult kidney, Hnf4α is expressed at a high level in proximal tubules. Although its expression begins from the embryonic period, its function in the developing kidney has remained almost unknown. In this study, we investigated the role of Hnf4α in the cultured embryonic mouse kidney by gene silencing using the RNA interference method. Additionally, we identified the dynamics of Hnf4α in the microenvironment of the developing kidney. As a result of gene silencing, the cellular organization in the condensed mesenchyme (CM) fell into disorder and many apoptotic cells appeared. In addition, laser microdissection‐reverse transcriptase‐polymerase chain reaction provided evidence that Hnf4α gene expression began first in the CM. These results suggest the possibility that Hnf4α plays an important role in the regulation of the cell survival at the CM stage in nephrogenesis. Developmental Dynamics 239:1145–1154, 2010. © 2010 Wiley‐Liss, Inc.
- Hokkaido Bunkyo University Japan
- Hokkaido University Japan
Mice, Knockout, Cell Survival, Organogenesis, Gene Expression Regulation, Developmental, Apoptosis, Kidney, Models, Biological, Mesoderm, Mice, Inbred C57BL, Mice, Organ Culture Techniques, Animals, Newborn, Hepatocyte Nuclear Factor 4, NIH 3T3 Cells, Animals, RNA Interference, RNA, Small Interfering
Mice, Knockout, Cell Survival, Organogenesis, Gene Expression Regulation, Developmental, Apoptosis, Kidney, Models, Biological, Mesoderm, Mice, Inbred C57BL, Mice, Organ Culture Techniques, Animals, Newborn, Hepatocyte Nuclear Factor 4, NIH 3T3 Cells, Animals, RNA Interference, RNA, Small Interfering
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).14 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Average influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Average impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!