The dataset contains records of Vascular Flora and Habitats collected the scope of the Complementary Studies (2010-11) and the baseline year (i.e., before construction) of the Ecological Monitoring Program of the Environmental Impact Assessment of the Foz Tua Hydroelectric Dam, promoted by EDP – Energias de Portugal, S.A. Data were collected in the spring 2011, during field visits to 24 sampling points, distributed along the river Tua valley, from the mouth to 52 km upstream.