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Análisis genómico de los daños por frío en tomate MicroTom

Genomic analysis of chilling injury in MicroTom fruits
Authors: Weiss, Julia Rosl; Fernández Trujillo, Juan Pablo; Gómez di Marco, Perla Azucena; Ferrer Ayala, María Ángeles; Calderón García, Antonio Asensio; Artés Calero, Francisco; Egea Gutiérrez-Cortines, Marcos;

Análisis genómico de los daños por frío en tomate MicroTom

Abstract

[SPA] La respuesta al frío en las plantas se ha identificado como un proceso de aclimatación que requiere la activación y represión de genes que permiten una mejor supervivencia. Dicha respuesta se produce en tiempos cortos o muy cortos de veinte a treinta minutos, lo que implica que los sensores de frío se encuentran presentes en las células vegetales responsables de la respuesta. Durante el proceso de comercialización, muchas frutas y hortalizas se almacenan en cámaras frigoríficas que reducen la velocidad de maduración o senescencia, pero que provocan en algunos casos daños por frío. Hemos utilizado el fruto de tomate de la variedad Micro Tom para llevar a cabo un cribado exhaustivo de genes activados y reprimidos por el frío. Sorprendentemente, ninguno de los genes que aparecieron en el cribado han sido identificados como genes de respuesta al frío o de maduración lo que sugiere que los daños por frío se deben a una disrupción de la maquinaria de mantenimiento celular. El gen LeCBF1 es un factor de transcripción que activa una de las rutas de aclimatación al frío. Dicho gen es activado por frío en hojas de tomate, pero no fue activado por el frío en frutos, lo que sugiere que la enorme sensibilidad de los frutos de tomate al frío puede deberse a una falta de respuesta a dicho estrés abiótico. [ENG] Cold sensing in plants has been described as an acclimation process that requires activation and repression of several sets of genes that improve plant survival. This response happens in short or very short periods between twenty and thirty minutes, suggesting that cold sensors are present in plant cells responsible of cold sensing and responses. During shipping and marketing, many fruits and vegetables are stored in cold chambers in order to reduce ripening or senescence speed, causing in some cases the so called chilling injury syndrome. We have used tomato fruits of the Microtom cultivar in order to perform and exhaustive screen for genes activated and repressed by the cold. Surprisingly none of the genes that appeared in the screen had been previously identified as cold-response or ripening genes suggesting that chilling injuries are due to a disruption of the house-keeping cellular system. The LeCBf1 gene is a transcription factor that activates one of the cold acclimation pathways. Gene expression analysis showed that cold treatment activated LeCBF1in leaves but not in fruits suggesting that the extreme sensitivity of tomato fruits to cold are due to a lack of response to this abiotic stress.

Country
Spain
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Keywords

Genómica funcional, 5102.01 Agricultura, LecBF1, Genotecas normalizadas, Functional genomics, Tecnología de los Alimentos, AITEP, Normalized cDNA libraries, Genética

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selected citations
These citations are derived from selected sources.
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
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