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EMBO Reports
Article . 2004 . Peer-reviewed
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A test of the CoHR motif associated with meiotic double‐strand breaks in Saccharomyces cerevisiae

Authors: Josep M. Comeron; Stuart J. Haring; Lucas J Lautner; Robert E. Malone;

A test of the CoHR motif associated with meiotic double‐strand breaks in Saccharomyces cerevisiae

Abstract

Meiotic recombination is not random along chromosomes; rather, there are preferred regions for initiation called hotspots. Although the general properties of meiotic hotspots are known, the requirements at the DNA sequence level for the determination of hotspot activity are still unclear. The sequence of six known hotspots in Saccharomyces cerevisiae was compared to identify a common homology region (CoHR). They reported that the locations of CoHR sequences correspond to mapped double‐strand break (DSB) sites along three chromosomes (I, III, VI). We report here that a deletion of CoHR at HIS2, a hotspot used to identify the motif, has no significant effect on recombination. In the absence of CoHR, DSB formation occurs at a high frequency and at the same sequences as in wild‐type strains. In cases where the deletion of sequences containing the CoHR motif has been shown to reduce recombination, we propose that it may be a reflection of the location of the deletion, rather than the loss of CoHR, per se.

Related Organizations
Keywords

Recombination, Genetic, Base Sequence, Amino Acid Motifs, Molecular Sequence Data, Chromosome Breakage, Saccharomyces cerevisiae, Meiosis, Sequence Homology, Nucleic Acid, Consensus Sequence, Chromosomes, Fungal, DNA, Fungal, Sequence Deletion

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
19
Average
Average
Top 10%
gold