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Fluorescent (C)LSM image sequences of Dictyostelium discoideum (Ax2 - LifeAct mRFP) for cell track and cell contour analysis

Name: Fluorescent (C)LSM image sequences of Dictyostelium discoideum (Ax2 - LifeAct mRFP) for cell track and cell contour analysis
Keywords: cell contour, cell tracking, Biophysics, confocal laser scanning microscopy (CLSM), Dictyostelium, fluorescent imaging

Research datakeyboard_double_arrow_right Dataset 11 Sep 2020Embargo end date: 11 Sep 2020 English Publisher:Dryad

Authors: Stange, Maike; Moldenhawer, Ted; Beta, Carsten;

doi: 10.5061/dryad.b5mkkwhbd

Fluorescent (C)LSM image sequences of Dictyostelium discoideum (Ax2 - LifeAct mRFP) for cell track and cell contour analysis

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Abstract

Fluorescent images sequences are recorded with a confocal laser scanning microscop. The sequences have the length of 500 and 1000 frames. The time interval is one second. All images have a resolution of 512 x 512 pixel and are 8 bit. The physical resolution is 134.95 x 134.95 µm and 212.55 x 212.55 µm. In preparation of the experiments the cells are staved for 4.5 hours in buffer to induce chemotaxis. Actin polymerization is visualized by using actin binding LifeAct-mRFP.

This data set is designed for cell contour and cell track analysis. Hence image sequences of moving Dictyostelium discoideum cells are recorded. For the purpose to facilitate the detection of the cell contour we take fluorescent images of the cortical protein actin to obtain a high contrast between background and cell body. In each image sequences several cells are recorded. This allows for an analysis of all cells at once or to crop single cell tracks.

The data format is accessible with the free software ImageJ (Fiji) and with Zeis Zen software (there is a limited free version available).

Related Organizations

University of Potsdam
Germany

Keywords

cell contour, cell tracking, Biophysics, confocal laser scanning microscopy (CLSM), Dictyostelium, fluorescent imaging

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