Identification of direct and indirect targets of the Gln3 and Gat1 activators by transcriptional profiling in response to nitrogen availability in the short and long term
Copyright policy )Identification of direct and indirect targets of the Gln3 and Gat1 activators by transcriptional profiling in response to nitrogen availability in the short and long term
Nitrogen catabolite repression (NCR) consists in the specific inhibition of transcriptional activation of genes encoding the permeases and catabolic enzymes needed to degrade poor nitrogen sources. Under nitrogen limitation or rapamycin treatment, NCR genes are activated by Gln3 or Gat1, or by both factors. To compare the sets of genes responding to rapamycin or to nitrogen limitation, we used DNA microarrays to establishing the expression profiles of a wild type strain, and of a double gln3Delta-gat1Delta strain, grown on glutamine, after addition of rapamycin, on proline, or after a shift from glutamine to proline. Analysis of microarray data revealed 392 genes whose expression was dependent on the nitrogen source quality. 91 genes were activated in a GATA factor-dependent manner in all growth conditions, suggesting a direct role of Gln3 and Gat1 in their expression. Other genes were only transiently up-regulated (stress-responsive genes) or down-regulated (genes encoding ribosomal proteins and translational factors) upon nitrogen limitation, and this regulation was delayed in a gln3Delta-gat1Delta strain. Repression of amino acid and nucleotide biosynthetic genes after a nitrogen shift did not depend on Gcn4. Several transporter genes were repressed as a consequence of enhanced levels of NCR-responsive permeases present at the plasma membrane.
- Université Libre de Bruxelles Belgium
Ribosomal Proteins, Ribosomal Proteins -- genetics, Saccharomyces cerevisiae Proteins, Prions -- physiology, Proline, Nitrogen, Prions, Glutamine, Repressor Proteins -- physiology, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins -- physiology, GATA Transcription Factors, Promoter Regions, Saccharomyces cerevisiae -- genetics, Genetic, Gene Expression Regulation, Fungal, GATA Transcription Factors -- metabolism, GATA Transcription Factors -- physiology, Promoter Regions, Genetic, Saccharomyces cerevisiae -- metabolism, Proline -- metabolism, Oligonucleotide Array Sequence Analysis, Transcription Factors -- physiology, Sirolimus, Glutathione Peroxidase, Binding Sites, Gene Expression Profiling, Sciences bio-médicales et agricoles, Repressor Proteins, Fungal, Gene Expression Regulation, Nitrogen -- metabolism, Sirolimus -- pharmacology, Glutamine -- metabolism, Transcription Factors
Ribosomal Proteins, Ribosomal Proteins -- genetics, Saccharomyces cerevisiae Proteins, Prions -- physiology, Proline, Nitrogen, Prions, Glutamine, Repressor Proteins -- physiology, Saccharomyces cerevisiae, Saccharomyces cerevisiae Proteins -- physiology, GATA Transcription Factors, Promoter Regions, Saccharomyces cerevisiae -- genetics, Genetic, Gene Expression Regulation, Fungal, GATA Transcription Factors -- metabolism, GATA Transcription Factors -- physiology, Promoter Regions, Genetic, Saccharomyces cerevisiae -- metabolism, Proline -- metabolism, Oligonucleotide Array Sequence Analysis, Transcription Factors -- physiology, Sirolimus, Glutathione Peroxidase, Binding Sites, Gene Expression Profiling, Sciences bio-médicales et agricoles, Repressor Proteins, Fungal, Gene Expression Regulation, Nitrogen -- metabolism, Sirolimus -- pharmacology, Glutamine -- metabolism, Transcription Factors
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).73 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!