DeepCAGE Transcriptomics Reveal an Important Role of the Transcription Factor MAFB in the Lymphatic Endothelium
Copyright policy )DeepCAGE Transcriptomics Reveal an Important Role of the Transcription Factor MAFB in the Lymphatic Endothelium
VEGF-C/VEGFR-3 signaling plays a central role in lymphatic development, regulating the budding of lymphatic progenitor cells from embryonic veins and maintaining the expression of PROX1 during later developmental stages. However, how VEGFR-3 activation translates into target gene expression is still not completely understood. We used cap analysis of gene expression (CAGE) RNA sequencing to characterize the transcriptional changes invoked by VEGF-C in LECs and to identify the transcription factors (TFs) involved. We found that MAFB, a TF involved in differentiation of various cell types, is rapidly induced and activated by VEGF-C. MAFB induced expression of PROX1 as well as other TFs and markers of differentiated LECs, indicating a role in the maintenance of the mature LEC phenotype. Correspondingly, E14.5 Mafb−/− embryos showed impaired lymphatic patterning in the skin. This suggests that MAFB is an important TF involved in lymphangiogenesis.
Cell Reports, 13 (7)
ISSN:2666-3864
ISSN:2211-1247
- University of Tsukuba Japan
- University of California System United States
- ETH Zurich Switzerland
- CLS Technology (Norway) Norway
- Institute of Pharmaceutical Sciences Switzerland
Male, Transcriptional Activation, QH301-705.5, MafB Transcription Factor, Molecular Sequence Data, Embryonic Development, Mice, Transgenic, Animals, Humans, Biology (General), Lymphangiogenesis, Promoter Regions, Genetic, Cells, Cultured, Lymphatic Vessels, Binding Sites, Base Sequence, Gene Expression Profiling, Cell Differentiation, Antigens, Differentiation, Mice, Inbred C57BL, Endothelium, Lymphatic, Protein Binding
Male, Transcriptional Activation, QH301-705.5, MafB Transcription Factor, Molecular Sequence Data, Embryonic Development, Mice, Transgenic, Animals, Humans, Biology (General), Lymphangiogenesis, Promoter Regions, Genetic, Cells, Cultured, Lymphatic Vessels, Binding Sites, Base Sequence, Gene Expression Profiling, Cell Differentiation, Antigens, Differentiation, Mice, Inbred C57BL, Endothelium, Lymphatic, Protein Binding
citations This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).46 popularity This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.Top 10% influence This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).Top 10% impulse This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.Top 10%
Citations provided by BIP!Popularity provided by BIP!