Diffuse large B cell lymphoma (DLBCL) is the most predominant type of Non Hodgkin- Lymphomas, representing more than one third of all recently diagnosed cases. Long non-coding RNAs (lncRNAs) are a class of RNA not coding for protein having more than 200 nucleotides and have significant roles in tumor formation and they have been investigated in different types of solid and hematologic tumors, including DLBCL.This work aims to study the level of lncRNAs(NAALADL2 and XIST)gene expression in DLBCL.NAALADL2 and XIST gene expression levels in blood were performed by real time qPCR technique. Forty five DLBCL patients and forty five age and gender matched healthy controls were included in this study.There was significant statistical increase in the expression of NAALADL2 and decrease of XIST expression in patients compared to control group (p value 10.3 and<1.57 for NAALADL2 and XISTrespectively.There was significant positive correlation between NAALADL2 and advanced stage and a significant negative correlation is found between XIST and advanced stage. It may be concluded that NAALADL2 and XISTexpression levels were obviously associated with DLBCL and might determine the disease progression.