Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale
Copyright policy )Bisulfite-free, base-resolution analysis of 5-formylcytosine at the genome scale
Active DNA demethylation in mammals involves oxidation of 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC), 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC). However, genome-wide detection of 5fC at single-base resolution remains challenging. Here we present fC-CET, a bisulfite-free method for whole-genome analysis of 5fC based on selective chemical labeling of 5fC and subsequent C-to-T transition during PCR. Base-resolution 5fC maps showed limited overlap with 5hmC, with 5fC-marked regions more active than 5hmC-marked ones.
- Howard Hughes Medical Institute United States
- Tsinghua University China (People's Republic of)
- Peking-Tsinghua Center for Life Sciences China (People's Republic of)
- UNIVERSITY OF CHICAGO
- Peking University China (People's Republic of)
Epigenomics, Genome, Stem Cells, Oligonucleotides, Mice, Transgenic, Sequence Analysis, DNA, DNA Methylation, Polymerase Chain Reaction, Article, Cell Line, Oxygen, Cytosine, Mice, Gene Expression Regulation, 5-Methylcytosine, Animals, Sulfites, CpG Islands, DNA Primers
Epigenomics, Genome, Stem Cells, Oligonucleotides, Mice, Transgenic, Sequence Analysis, DNA, DNA Methylation, Polymerase Chain Reaction, Article, Cell Line, Oxygen, Cytosine, Mice, Gene Expression Regulation, 5-Methylcytosine, Animals, Sulfites, CpG Islands, DNA Primers
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