Additional file 1: Figure S1: Absence of GCI pathology in the cerebellar cortex of MSA-C patients. a: Graphical overview illustrating regions of interest within the human cerebellum including the granular cell layer (GCL) and molecular layer (ML). b, c: Co-staining of OLIG2 (brown) and α-syn (red) within the ML (b) and GCL (c). Nuclei were counterstained using haematoxylin (blue). Inserts show OLIG2+ oligodendrocytes. Scale bar: 20 µm. Figure S2: Expression of human alpha-synuclein (α-syn) predominantly within the cerebellar white matter of MBP29-hα-syn (MBP29) mice. a: Immunofluorescence staining for OLIG2 (magenta) as oligodendrocyte-specific marker and α-syn (green) within the cerebellar white matter (cbw) and cerebellar cortex (cbx) of 8- and 16-week-old MBP29-hα-syn mice compared to ntg mice; scale bar: 50 µm. b: Co-staining of phosphorylated α-syn (pS129-α-syn; magenta) and α-syn (green) within the cbw and cbx of 8- and 16-week-old MBP29-hα-syn mice compared to ntg mice; scale bar: 10 µm. c: Cellular expression of TPPP/p25α (magenta) and α-syn (green) within cbw and cbx of 8- and 16-week-old MBP29-hα-syn mice compared to ntg mice; scale bar: 10 µm. All images are shown as maximum intensity projection images; DAPI+ nuclei are shown in blue. Table S1: Changes of gait parameters and bodyweight in MBP29-hα-syn mice (MBP29) vs. non-transgenic controls (ntg). Table S2: Subgroup gait analysis in MBP29-hα-syn (MBP29) mice (completed) compared to MBP29-hα-syn mice (non-completed).