Prothymosin α associates with the oncoprotein SET and is involved in chromatin decondensation
Copyright policy )Prothymosin α associates with the oncoprotein SET and is involved in chromatin decondensation
Prothymosin α (ProTα) is a histone H1‐binding protein that interacts with the transcription coactivator CREB‐binding protein and potentiates transcription. Based on coimmunoprecipitation and mammalian two‐hybrid assays, we show here that ProTα forms a complex with the oncoprotein SET. ProTα efficiently decondenses human sperm chromatin, while overexpression of GFP–ProTα in mammalian cells results in global chromatin decondensation. These results indicate that decondensation of compacted chromatin fibers is an important step in the mechanism of ProTα function.
- European Molecular Biology Laboratory Germany
- University of Ioannina Greece
- University of Konstanz Germany
- European Bioinformatics Institute United Kingdom
Cell Extracts, Male, Silver Staining, Chromosomal Proteins, Non-Histone, Blotting, Western, Green Fluorescent Proteins, Molecular Sequence Data, Proteins/chemistry/*metabolism, Luciferases/metabolism, Oncoprotein SET, Mass Spectrometry, Protein Precursors/genetics/*metabolism, Chromatin/*metabolism, Sequence Analysis, Protein, Two-Hybrid System Techniques, Humans, Histone Chaperones, Amino Acid Sequence, Trans-Activators/metabolism, Luciferases, Green Fluorescent Proteins/metabolism, Nuclear Proteins/metabolism, Recombinant Proteins/metabolism, Chromatin decondensation, CREB-binding protein, Spermatozoa/metabolism, Nuclear Proteins, Prothymosin α, Plasmids/metabolism, Thymosin/*analogs & derivatives/genetics/*metabolism, CREB-Binding Protein, Precipitin Tests, Chromatin, DNA-Binding Proteins, Molecular Weight, Transcription Factors, HeLa Cells, Plasmids, Protein Binding
Cell Extracts, Male, Silver Staining, Chromosomal Proteins, Non-Histone, Blotting, Western, Green Fluorescent Proteins, Molecular Sequence Data, Proteins/chemistry/*metabolism, Luciferases/metabolism, Oncoprotein SET, Mass Spectrometry, Protein Precursors/genetics/*metabolism, Chromatin/*metabolism, Sequence Analysis, Protein, Two-Hybrid System Techniques, Humans, Histone Chaperones, Amino Acid Sequence, Trans-Activators/metabolism, Luciferases, Green Fluorescent Proteins/metabolism, Nuclear Proteins/metabolism, Recombinant Proteins/metabolism, Chromatin decondensation, CREB-binding protein, Spermatozoa/metabolism, Nuclear Proteins, Prothymosin α, Plasmids/metabolism, Thymosin/*analogs & derivatives/genetics/*metabolism, CREB-Binding Protein, Precipitin Tests, Chromatin, DNA-Binding Proteins, Molecular Weight, Transcription Factors, HeLa Cells, Plasmids, Protein Binding
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