Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis
Copyright policy )Ebola Virus VP35 Interaction with Dynein LC8 Regulates Viral RNA Synthesis
ABSTRACT Ebola virus VP35 inhibits alpha/beta interferon production and functions as a viral polymerase cofactor. Previously, the 8-kDa cytoplasmic dynein light chain (LC8) was demonstrated to interact with VP35, but the functional consequences were unclear. Here we demonstrate that the interaction is direct and of high affinity and that binding stabilizes the VP35 N-terminal oligomerization domain and enhances viral RNA synthesis. Mutational analysis demonstrates that VP35 interaction is required for the functional effects of LC8.
- Washington University in St. Louis United States
- University of Mary United States
- Washington University in St. Louis United States
- Icahn School of Medicine at Mount Sinai United States
- Washington University in St. Louis School of Medicine United States
Cytoplasmic Dyneins, DNA Mutational Analysis, Host-Pathogen Interactions, Protein Interaction Mapping, RNA, Viral, Viral Regulatory and Accessory Proteins, Ebolavirus, Virus Replication, Protein Binding
Cytoplasmic Dyneins, DNA Mutational Analysis, Host-Pathogen Interactions, Protein Interaction Mapping, RNA, Viral, Viral Regulatory and Accessory Proteins, Ebolavirus, Virus Replication, Protein Binding
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