A Pre-mRNA-Binding Protein Accompanies the RNA from the Gene through the Nuclear Pores and into Polysomes
Copyright policy )A Pre-mRNA-Binding Protein Accompanies the RNA from the Gene through the Nuclear Pores and into Polysomes
In the larval salivary glands of C. tentans, it is possible to visualize by electron microscopy how Balbiani ring (BR) pre-mRNA associates with proteins to form pre-mRNP particles, how these particles move to and through the nuclear pore, and how the BR RNA is engaged in the formation of giant polysomes in the cytoplasm. Here, we study C. tentans hrp36, an abundant protein in the BR particles, and establish that it is similar to the mammalian hnRNP A1. By immuno-electron microscopy it is demonstrated that hrp36 is added to BR RNA concomitant with transcription, remains in nucleoplasmic BR particles, and is translocated through the nuclear pore still associated with BR RNA. It appears in the giant BR RNA-containing polysomes, where it remains as an abundant protein in spite of ongoing translation.
- Karolinska Institute Sweden
Cell Nucleus, Cytoplasm, Sequence Homology, Amino Acid, Biochemistry, Genetics and Molecular Biology(all), Nuclear Envelope, Heterogeneous Nuclear Ribonucleoprotein A1, Molecular Sequence Data, RNA-Binding Proteins, Biological Transport, Genes, Insect, Sequence Analysis, DNA, Chironomidae, Heterogeneous-Nuclear Ribonucleoproteins, Salivary Glands, Ribonucleoproteins, Polyribosomes, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, RNA Precursors, Animals, Amino Acid Sequence, Cloning, Molecular
Cell Nucleus, Cytoplasm, Sequence Homology, Amino Acid, Biochemistry, Genetics and Molecular Biology(all), Nuclear Envelope, Heterogeneous Nuclear Ribonucleoprotein A1, Molecular Sequence Data, RNA-Binding Proteins, Biological Transport, Genes, Insect, Sequence Analysis, DNA, Chironomidae, Heterogeneous-Nuclear Ribonucleoproteins, Salivary Glands, Ribonucleoproteins, Polyribosomes, Heterogeneous-Nuclear Ribonucleoprotein Group A-B, RNA Precursors, Animals, Amino Acid Sequence, Cloning, Molecular
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