Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization
Copyright policy )Identification of Thermotolerant Campylobacter Species by Fluorescence In Situ Hybridization
ABSTRACT Thermotolerant Campylobacter spp. ( Campylobacter jejuni , C. coli , C. lari , and C. upsaliensis ) are leading causes of food-borne diarrhea in humans. In this study, the usefulness of fluorescence in situ hybridization (FISH) for the identification of Campylobacter isolates was investigated. A hierarchical FISH probe set that included six group-, genus-, and species-specific probes was developed and evaluated with 12 reference strains and 94 clinical isolates of Campylobacter , Arcobacter , and Helicobacter . FISH correctly identified all isolates to the genus level and detected all thermotolerant Campylobacter isolates. The assay showed high degrees of sensitivity for the identification of C. jejuni (90%), C. coli (97%), C. lari (81%), and C. upsaliensis (100%) to the species level.
- University of Ulm Germany
- Institute of Medical Microbiology and Hygiene Germany
- Federal Institute for Risk Assessment Germany
Hot Temperature, Species Specificity, Humans, Campylobacter, DNA Probes, In Situ Hybridization, Fluorescence, Bacterial Typing Techniques, Culture Media
Hot Temperature, Species Specificity, Humans, Campylobacter, DNA Probes, In Situ Hybridization, Fluorescence, Bacterial Typing Techniques, Culture Media
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