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European Journal of Biochemistry
Article
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European Journal of Biochemistry
Article . 1980 . Peer-reviewed
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European Journal of Biochemistry
Article . 1981
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NADP‐Dependent Isocitrate Dehydrogenase from the Mussel Mytilus edulis L.

1. Purification and Characterisation
descriptionPublicationkeyboard_double_arrow_right Article 01 Oct 1980Publisher:WileyJournal:European Journal of Biochemistry, volume 111, pages 575-579 (issn: 0014-2956, eissn: 1432-1033, Copyright policy )
Authors: Erica J. H. Head;

doi: 10.1111/j.1432-1033.1980.tb04973.x

pmid: 7460916

NADP‐Dependent Isocitrate Dehydrogenase from the Mussel Mytilus edulis L.

Abstract

A purification scheme is described for NADP‐dependent isocitrate dehydrogenase front the digestive gland of Mytilus edulis. The scheme incorporates three chromatographic steps: hydroxyapatite adsorption, blue‐Sepharose affinity chromatography and DEAE‐Sephacel ion‐exchange chromatography. The subunit molecular weight of the enzyme was 45000 (± 5000) by sodium dodecylsulfate gel electrophoresis. The purified enzyme was homogeneous on dodecylsulfate gels and had a specific activity of 30–50 U/mg protein.

Related Organizations
Keywords

Molecular Weight, Macromolecular Substances, Animals, Digestive System, Chromatography, Affinity, Isocitrate Dehydrogenase, NADP, Bivalvia

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citations
This is an alternative to the "Influence" indicator, which also reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Citations provided by BIP!
popularity
This indicator reflects the "current" impact/attention (the "hype") of an article in the research community at large, based on the underlying citation network.
BIP!Popularity provided by BIP!
influence
This indicator reflects the overall/total impact of an article in the research community at large, based on the underlying citation network (diachronically).
BIP!Influence provided by BIP!
impulse
This indicator reflects the initial momentum of an article directly after its publication, based on the underlying citation network.
BIP!Impulse provided by BIP!
10
Average
Average
Average
bronze