descriptionPublicationkeyboard_double_arrow_right Article 01 Jun 1984 English Publisher:Elsevier BVJournal:Gene, volume 28, pages 331-335 (issn: 0378-1119,

Authors: B E, Froman; R C, Tait; C I, Kado; R L, Rodriguez;

doi: 10.1016/0378-1119(84)90150-1

pmid: 6086461

Purification of restriction endonuclease XcyI from Xanthomonas cyanopsidis

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Abstract

A new Type II restriction endonuclease XcyI, purified from Xanthomonas cyanopsidis 13D5, is an isoschizomer of SmaI and XmaI that cleaves at the nucleotide sequence 5'-C decreases CCGGG-3' of double-stranded DNA. The single restriction activity present in this strain permits rapid purification of 8000 units of cleavage activity from 10 g of freshly harvested cells. The resulting XcyI preparation is free of contaminating nuclease activities that interfere with in vitro manipulation of DNA.

Related Organizations

University of California, Davis
United States

Keywords

DNA, Bacterial, Xanthomonas, Bacterial Proteins, Base Sequence, DNA Restriction Enzymes

1 Research products, page 1 of 1

Structure and evolution of theXcylrestriction-modification system
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