Copyright information:Taken from "Crystal structure and RNA binding of the Rpb4/Rpb7 subunits of human RNA polymerase II"Nucleic Acids Research 2005;33(19):6435-6444.Published online 10 Nov 2005PMCID:PMC1283528.© The Author 2005. Published by Oxford University Press. All rights reserved () Example of a mobility assay using the E/F heterodimer. Lane 1, free probe; lanes 2–8, increasing concentrations of the E/F complex with a F95A mutation in the E subunit; lanes 10–15, increasing concentrations of the E/F complex with a D105K mutation in the E subunit. () Example of a mobility assay using the E/F heterodimer. Lane 1, free probe; lanes 2-8, increasing concentrations of the E/F complex with a Q112K mutation in the E subunit; lanes 10–15, increasing concentrations of the E/F complex with the Δloop mutation in the E subunit. () Summary of the effect of mutations in the E subunit on the RNA-binding properties. For each mutant protein, the percentage of RNA probe bound to the protein, after background correction, is shown. () Example of a mobility assay performed using the human Rpb4/Rpb7 heterodimer. Lane 1, free probe; lanes 2–7, increasing concentrations of wild-type Rpb4/Rpb7 (ranging from 125 ng to 10 µg); lanes 9–14, increasing concentrations of Rpb4/Rpb7 with a H14E mutation in Rpb7. () Example of a mobility assay performed using the human Rpb4/Rpb7 heterodimer. Lane 1, free probe; lanes 2-8, increasing concentrations of Rpb4/Rpb7 with a D153K mutation in Rpb7; lanes 10–15, increasing concentrations of Rpb4/Rpb7 with a F158A mutation in Rpb7. () Summary of the effect of mutations in the human Rpb7 subunit on the RNA-binding properties, where experimental data were treated as described for the E subunit.