Two R7 Regulator of G-Protein Signaling Proteins Shape Retinal Bipolar Cell Signaling
Copyright policy )Two R7 Regulator of G-Protein Signaling Proteins Shape Retinal Bipolar Cell Signaling
RGS7, RGS11, and their binding partner Gbeta5 are localized to the dendritic tips of retinal ON bipolar cells (ON-BPC), where mGluR6 responds to glutamate released from photoreceptor terminals by activation of the RGS7/RGS11 substrate, Galphao. To determine their functions in retinal signaling, we investigated cell-specific expression patterns of RGS7 and RGS11 by immunostaining, and measured light responses by electroretinography in mice with targeted disruptions of the genes encoding them. RGS7 staining is present in dendritic tips of all rod ON-BPC, but missing in those for subsets of cone ON-BPC, whereas the converse was true for RGS11 staining. Genetic disruption of either RGS7 or RGS11 produced delays in the ON-BPC-derived electroretinogram b-wave, but no changes in the photoreceptor-derived a-wave. Homozygous RGS7 mutant mice had delays in rod-driven b-waves, whereas RGS11 mutant mice had delays in rod-driven, and especially in cone-driven b-waves. The b-wave delays were further enhanced in mice homozygous for both RGS7 and RGS11 gene disruptions. Thus, RGS7 and RGS11 act in parallel to regulate the kinetics of ON bipolar cell responses, with differential impacts on the rod and cone pathways.
- Baylor College of Medicine United States
Retinal Bipolar Cells, Light, Adaptation, Ocular, GTP-Binding Protein beta Subunits, GTPase-Activating Proteins, Dendrites, GTP-Binding Protein alpha Subunits, Gi-Go, Receptors, Metabotropic Glutamate, Mice, Mutant Strains, Retina, Mice, Inbred C57BL, Electrooculography, Mice, Gene Expression Regulation, Animals, Eye Proteins, Evoked Potentials, Protein Kinase C, RGS Proteins, Signal Transduction
Retinal Bipolar Cells, Light, Adaptation, Ocular, GTP-Binding Protein beta Subunits, GTPase-Activating Proteins, Dendrites, GTP-Binding Protein alpha Subunits, Gi-Go, Receptors, Metabotropic Glutamate, Mice, Mutant Strains, Retina, Mice, Inbred C57BL, Electrooculography, Mice, Gene Expression Regulation, Animals, Eye Proteins, Evoked Potentials, Protein Kinase C, RGS Proteins, Signal Transduction
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