The significance of tocopherols as chemotaxonomic markers and their relationship withoil content and fatty acid profile was investigated in a collection of 91 species of the familyBrassicaceae. Total tocopherols content ranged from 68 mg kg−1 oil in Diplotaxis viminea to2479 mg kg−1 oil in Schivereckia doerfleri. The collection also showed wide variability fortocopherol composition. The average tocopherol profile consisted of 65.4% g-, 28.7% a-, 5.1%d- and 0.8% β-tocopherol. Individual tocopherols were found to have great taxonomic value inthe Brassicaceae.